<i>Lactobacillus rhamnosus</i>-derived extracellular vesicles influence calcium deposition in a model of breast cancer intraductal calcium stress.

ABSTRACT: Extracellular calcium export by the breast ductal epithelium is crucial during lactation and plays a significant role in breast cancer progression. Intraductal calcium deposition is a hallmark of aggressive premalignant lesions. This study tested the hypothesis that microbiome-derived extracellular vesicles (EVs) influence calcium modulation in premalignant breast cancer lesions. Based on the analysis of plasma, serum, saliva, and tissue collected from breast cancer patients and controls (N = 150), Lactobacillus rhamnosus (Lr) was chosen as the model microbiota. In a BT-474 human breast cancer cell line monolayer culture under acute calcium stress, Lr EVs enhanced intracellular calcium intake. In a BT474 3D spheroid model under chronic calcium stress, Lr

SUBMITTER: Vuong N 

PROVIDER: S-EPMC12148604 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature