Metabolomic and Genomic Analysis of Bioactive Compounds of Phacidium infestans Karsten DSM 5139 Cultivated on Pinus sylvestris Needles.

ABSTRACT: This study investigates how Phacidium infestans acquires nutrients on Pinus sylvestris needles, which possess antimicrobial properties. P. infestans was evaluated for its growth and enzyme production on various substrates, alongside genomic and metabolomic analysis. Direct-infusion high-resolution mass spectrometry (DI-HRMS) was performed on methanol extracts obtained from P. infestans cultivated on needles and malt extract media. DI-HRMS analysis identified 21 compounds from the malt extract and 112 from the needle samples. The resin components increased in the needle samples post-cultivation, suggesting terpenoid release from resin ducts due to fungal degradation of plant cell walls. P. infestans fully consumed sugars and antifungal compounds, including taxiresinol and salicylic acid, wi

SUBMITTER: Zerouki C 

PROVIDER: S-EPMC12148953 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature