Dose-dependent immunotoxic mechanisms of celastrol via modulation of the PI3K-Akt signaling pathway.

ABSTRACT:

Ethnopharmacological relevance

Celastrol, a bioactive compound from Tripterygium wilfordii Hook.f., is known for its anti-inflammatory and immunomodulatory effects, but its immunotoxicity is underexplored. This study investigates the mechanisms of celastrol-induced immunotoxicity, focusing on the PI3K-Akt signaling pathway, a key regulator of immune function.

Materials and methods

An integrative approach combining network toxicology, molecular docking, and experimental biology identified molecular targets in celastrol-induced immune dysfunction. Network toxicology mapped key pathways, and molecular docking predicted interactions with immune-related proteins. High-dose celastrol (10 mg/kg) was administered to C57BL/6J mice, followed by a histopathological analysis of t

SUBMITTER: Geng S 

PROVIDER: S-EPMC12149175 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature