Crystal Structure of Glycoside Hydrolase Family 20 Lacto-<i>N</i>-biosidase from Soil Bacterium <i>Streptomyces</i> sp. Strain 142.

ABSTRACT: Lacto-N-biosidase hydrolyzes the β-GlcNAc or β-GalNAc bond of sugar chains to release lacto-N-biose I (Gal-β1,3-GlcNAc) or galacto-N-biose (Gal-β1,3-GalNAc) from the non-reducing end. Typical substrates for lacto-N-biosidase include type I oligosaccharides contained in human breast milk, such as lacto-N-tetraose. Lacto-N-biosidases have recently received significant attention because of their potential to synthesize milk oligosaccharides. Bifidobacterial lacto-N-biosidases belonging to glycoside hydrolase families 20 and 136 have been studied. The GH20 lacto-N-biosidases utilize a substrate-associated hydrolysis mechanism. LnbB from Bifidobacterium bifidum is the only lacto-N-biosidase with reported crystal structures in

SUBMITTER: Fujio N 

PROVIDER: S-EPMC12149517 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature