Crystal Structure of CcGH131B, a Protein Belonging to Glycoside Hydrolase Family 131 from the Basidiomycete <i>Coprinopsis cinerea</i>.

ABSTRACT: Glycoside hydrolase family 131 (GH131) proteins are found in oomycetes, ascomycetes, and basidiomycetes, and have been reported to hydrolyze various β-glucan polysaccharides. Coprinopsis cinerea, a model basidiomycete, contains two GH131 proteins, CcGH131A and CcGH131B. This study focuses on the structural and functional properties of CcGH131B, a protein that lacks the carbohydrate bonding module 1 (CBM1) domain present in CcGH131A. The crystal structure of CcGH131B was determined. The structure displayed a β-jelly roll fold with extra loops and α-helices, resulting in a deeper substrate-binding groove compared to CcGH131A and also PaGluc131A, a GH131 protein from Podospora anserina. A cellobiose-bound structure of the E161A mutant, in which the potential catalytic residue Gl

SUBMITTER: Shiojima Y 

PROVIDER: S-EPMC12149522 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature