Structural studies on ribosomes of differentially macrolide-resistant <i>Staphylococcus aureus</i> strains.

ABSTRACT: Antimicrobial resistance is a major global health challenge, diminishing the efficacy of many antibiotics, including macrolides. In Staphylococcus aureus, an opportunistic pathogen, macrolide resistance is primarily mediated by Erm-family methyltransferases, which mono- or dimethylate A2058 in the 23S ribosomal RNA, reducing drug binding. Although macrolide-ribosome interactions have been characterized in nonpathogenic species, their structural basis in clinically relevant pathogens remains limited. In this study, we investigate the impact of ermB-mediated resistance on drug binding by analyzing ribosomes from S. aureus strains with varying levels of ermB expression and activity. Using cryo-electron microscopy, we determined the high-resolution structures of sol

SUBMITTER: Rivalta A 

PROVIDER: S-EPMC12149560 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature