Deciphering alternative splicing patterns during cell fate transition of fast chemical reprogramming.

ABSTRACT:

Background

Alternative splicing (AS) is a substantial contributor to the high complexity of transcriptomes in multicellular eukaryotes. Fast chemical reprogramming (FCR) system is an innovative approach that facilitates the rapid transition of somatic cells into induced pluripotent stem cells (iPSCs).

Results

In this study, we used the FCR system to delve into the dynamics of AS during cell fate transition. The trajectory of FCR, as characterized by gene expression profiles, consistently aligned with that observed in AS patterns, revealing a complex interplay between AS and gene expression regulation. Additionally, we discovered that the exon exclusion events were more prevalent than the exon inclusion events, indicating a predominant mode of splicing regulation during FCR.

SUBMITTER: Lu Y 

PROVIDER: S-EPMC12150459 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature