Evidence for a model of conformational change by the <i>Plasmodium falciparum</i> circumsporozoite protein during sporozoite development in the mosquito host through the use of camelid single-domain antibodies.

ABSTRACT: Plasmodium sporozoites (SPZs) are formed in the Anopheles mosquito midgut from where they travel to the salivary glands and subsequently to the mammalian liver after deposition into the skin. The SPZ's main surface antigen, the circumsporozoite protein (CSP), plays a pivotal role in SPZ biology and constitutes the immunodominant target for host antibodies. In this study, we raised single-domain antibodies (sdAbs) against CSP from P. falciparum (PfCSP) by immunizing two alpacas with recombinant versions of the antigen. We found that all identified sdAbs specifically target PfCSP's globular [Formula: see text]TSR domain without cross-reacting with P. berghei CSP. Further characterization revealed that most sdAbs recognize native PfCSP on the SPZ surface, although

SUBMITTER: Geens R 

PROVIDER: S-EPMC12150756 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature