VSOT: volume-surface optimization for accurate ultrastructure analysis of dendritic spines.

ABSTRACT:

Motivation

Morphological analysis of dendritic spines is critical to understanding the function and dysfunction of neural circuits. The growing trends of the large-scale electron microscopy (EM) imaging systems and automatic cellular reconstruction provide unprecedented opportunities to investigate the ultrastructure of dendrites. This morphometric analysis of dendritic spines requires accurate compartment segmentation methods as well as meaningful quantification methods. However, most existing methods rely on surface or volumetric information alone, which may not deliver accurate segmentation results.

Results

We developed VSOT, a method based on Volume-Surface Optimization, designed for the accurate structural analysis of dendritic reconstruction. VSOT accurately segments d

SUBMITTER: Lyu B 

PROVIDER: S-EPMC12150773 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature