Formation of chromosomal rearrangements in <i>Saccharomyces cerevisiae</i> diploids through regionally-biased non-allelic homologous recombination.

ABSTRACT: In earlier studies, we optimized an assay system for the genome-wide detection of copy number variation (CNV) in diploid Saccharomyces cerevisiae cells, based on selection for formaldehyde plus copper (FA+Cu) resistance conferred by the amplification of a dosage-dependent reporter cassette, SFA1-CUP1. Our analyses identified a robust bias for terminal deletions of the right arm of Chr7 (Chr7R) associated with unbalanced translocations. This bias was observed at approximately constant strength across all three sites where the amplification reporter cassette was inserted, in CNV-carrying yeast clones derived both spontaneously and from mutagen-induced recombinogenic conditions. We conducted allelic mitotic recombination experiments to investigate the possibility of the presence

SUBMITTER: Merriman SA 

PROVIDER: S-EPMC12151323 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature