Heterologous prime-boost vaccination drives stromal activation and adaptive immunity against SARS-CoV-2 variants.

ABSTRACT: Heterologous vaccination strategies have shown superior efficacy over homologous regimens in clinical studies, but the underlying immunological mechanisms remain incompletely understood. Using a mouse model, we investigated the immune responses induced by heterologous prime-boost vaccination with adenoviral and mRNA vaccines. Heterologous vaccination (adenoviral prime, mRNA boost) elicited higher neutralizing antibody titers and stronger CD8⁺ T cell responses against Delta and Omicron-BA.5 variants compared to homologous regimens. Single-cell transcriptomic analysis of injection-site tissues revealed that adenoviral priming induced minimal changes in cellular composition but established a pre-conditioned innate immune environment. This effect was further amplified upon mRNA boos

SUBMITTER: Jeon JH 

PROVIDER: S-EPMC12151836 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature