Lamin A/C promotes HBV transcription by modulating histone modification associated with cccDNA minichromosome in an HBx-dependent manner.

ABSTRACT:

Background

Chronic hepatitis B virus (HBV) infection remains a serious public health challenge, and primary treatment strategies are unable to cure HBV infection due to the persistence of HBV covalently closed circular DNA (cccDNA) in the nuclei of cells. The HBV X protein (HBx) plays a crucial role in regulating cccDNA transcription. Therefore, targeting HBx to identify host proteins that regulate cccDNA transcription could represent a curative approach.

Methods

Here, we used co-immunoprecipitation (co-IP) and mass spectrometry (MS) to identify proteins that interact with HBx. The candidate proteins Lamin A and C (Lamin A/C) were knocked down by RNA interference, and Lamin A/C was overexpressed via lentiviral vectors in HBV-infected cell cultures. Chromatin immunoprecipitat

SUBMITTER: Jiang L 

PROVIDER: S-EPMC12153130 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature