LINC02888 promotes HGSOC progression and immune evasion via PPIB-mediated stabilization of LAPTM5 mRNA and inhibition of RIG-I-like receptor signaling.

ABSTRACT:

Background

High-grade serous ovarian cancer (HGSOC) remains highly lethal due to late diagnosis and chemoresistance. Long noncoding RNAs (lncRNAs) have emerged as critical regulators of tumor progression. This study investigates the role of the cytoplasmic lncRNA LINC02888 in HGSOC.

Methods

We identified overexpression of LINC02888 in HGSOC tissues and cell lines through whole-transcriptome sequencing, qPCR, in situ hybridization, and analyses of TCGA/GTEx datasets. Functional assays including CCK-8, EdU, colony formation, wound healing, Transwell migration/invasion, and flow cytometry were performed after modulating LINC02888 expression. RNA pull-down coupled with mass spectrometry identified the RNA-binding protein PPIB as an interactor of LINC02888. RNA-seq following both

SUBMITTER: Rui B 

PROVIDER: S-EPMC12153195 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature