Detection of ESBL-producing <i>Klebsiella oxytoca</i> complex with VITEK 2 system and screening cutoffs for implementing confirmatory tests.

ABSTRACT: Klebsiella oxytoca complex (KoC) are important nosocomial pathogens that can be reservoirs of transmissible extended-spectrum β-lactamase (ESBL) genes. Therefore, it is essential for clinical microbiology laboratories to distinguish between KoC producing ESBLs (ESBL-KoC) and those hyperproducing the natural OXY-type β-lactamases (hOXY-KoC). We investigated the abilities of VITEK 2 with and without using the Advanced Expert System (AES) to detect ESBL producers among 44 well-characterized KoC strains (including 11 ESBL-KoC and 21 hOXY-KoC). VITEK 2/AES showed 100% sensitivity (Se) and 64.7% specificity (Sp), whereas the VITEK 2 coupled by the Clinical Laboratory Standards Institute (CLSI) ESBL confirmatory tests (ESBL-CTs; i.e., disk-c

SUBMITTER: Campos-Madueno EI 

PROVIDER: S-EPMC12153293 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature