The AusAB non-ribosomal peptide synthetase of <i>Staphylococcus aureus</i> preferentially generates phevalin in host-mimicking media.

ABSTRACT: Non-ribosomal peptide synthetases (NRPSs) are modular multidomain enzymes responsible for the biosynthesis of various secondary metabolites in an mRNA template-independent manner. They are predominantly present in bacteria and fungi, where they synthesize a variety of products, including antibiotics, siderophores, toxins, and signaling molecules. The human pathogen Staphylococcus aureus possesses one single NRPS, AusA, highly conserved in all sequenced S. aureus strains. AusA incorporates the aromatic amino acids (AAAs) phenylalanine or tyrosine, as well as the branched-chain amino acids valine and leucine into three cyclic dipeptides collectively called aureusimines: phevalin, tyrvalin, and leuvalin. By using targeted metabolomics, we found that during growth in the common t

SUBMITTER: Moldovan A 

PROVIDER: S-EPMC12153313 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature