Genome-wide maps of UV damage repair and mutation suppression by CPD photolyase.

ABSTRACT: Ultraviolet (UV) light causes cyclobutane pyrimidine dimers (CPDs) and other DNA lesions that must be efficiently repaired to prevent cell death and mutagenesis. While mammals utilize the nucleotide excision repair (NER) pathway to repair CPDs, many species primarily utilize photolyase enzymes to repair UV damage. Our understanding of how different genomic and chromatin features impact photolyase repair across a eukaryotic genome is limited. Here, we map repair of CPDs by photolyase across the yeast genome at single-nucleotide resolution. Our data indicate that yeast photolyase repairs CPDs more rapidly than NER, but photolyase activity is inhibited at certain classes of transcription factor binding sites and in nucleosomes. Repair in nucleosomes is particularly inhibited when CPDs are loc

SUBMITTER: Bohm KA 

PROVIDER: S-EPMC12153352 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature