Characterization of microRNA and Metabolite Profiles of Seminal Extracellular Vesicles in Boars.

ABSTRACT: Extracellular vesicles (EVs) contain bioactive substances and mediate a multitude of physiological functions. EVs can be found in most body fluids and are particularly abundant in semen. EVs have the potential to become a biomarker for the quality of boar semen. In this study, EVs were isolated from the semen of relatively young (10 months of age, Y-EVs) and old (30 months of age, O-EVs) duroc boars using ultracentrifugation. The isolated EVs were characterized using a transmission electron microscope, nanoparticle tracking analysis, and Western blotting. MicroRNA (miRNA) profiles and metabolomes were analyzed using high-throughput sequencing and liquid chromatography-mass spectrometry, respectively. The median particle sizes of Y-EVs and O-EVs were 151.3 nm and 162.1 nm, respectively. miR

SUBMITTER: Ma J 

PROVIDER: S-EPMC12153518 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature