Insulin-Degrading Enzyme Regulates mRNA Processing and May Interact with the CCR4-NOT Complex.

ABSTRACT: Insulin-degrading enzyme is a zinc metalloprotease that degrades low-molecular-weight substrates, including insulin. Ubiquitous expression, high evolutionary conservation, upregulation of Ide in stress situations, and literature findings suggest a broader function of Ide in cell physiology and protein homeostasis that remains to be elucidated. We used proteomics and transcriptomics approaches to search for leads related to a broader role of Ide in protein homeostasis. We combined an analysis of the proteome and single-cell transcriptome of Ide^+/+ and Ide^-/- pancreatic islet cells with an examination of the interactome of human cytosolic Ide using proximity biotinylation. We observe an upregulation of pathways related to RNA processing, translation and sp

SUBMITTER: Bertocci B 

PROVIDER: S-EPMC12153694 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature