Ovarian Stromal Cell-Conditioned Media, but Not Co-Culture, Improves Survival in Feline Follicles.

ABSTRACT: Ovarian stromal cells play roles in in vivo folliculogenesis; however, little is known about their effect on in vitro cultured follicles. This study investigated the impact of ovarian stromal cell co-culture or conditioned medium (CM) on the survival and development of domestic cat follicles in vitro. Preantral (n = 148 follicles), early antral (n = 92), and antral (n = 22) stage cat follicles were divided into five groups (control, ovarian stromal cell co-culture, 20% CM, 50% CM, and 100% CM), cultured for 13 days, and evaluated for survival, growth, and the mRNA expression of CYP19A, GDF9, and FSHR. Additional follicles (n = 199) were isolated, divided into three groups (control, co-culture, and 100% CM), cultured for 10 days, and oocytes were subjected to in vitro m

SUBMITTER: Marks B 

PROVIDER: S-EPMC12153732 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature