VPAC1 and VPAC2 Receptor Heterozygosity Confers Distinct Biological Properties to BV2 Microglial Cells.

ABSTRACT: Microglial cells, the resident immune cells of the central nervous system (CNS), are essential for maintaining CNS homeostasis. Dysregulation of microglial function is implicated in the pathogenesis of various neurodegenerative diseases. Vasoactive intestinal polypeptide receptors 1 and 2 (VPAC1 and VPAC2) are G-protein-coupled receptors (GPCRs) expressed by microglia, with their primary ligands being pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal peptide (VIP). However, the specific roles of VPAC-type receptors in microglial regulation remain poorly understood. In this study, we generated VPAC1^+/- and VPAC2^+/- BV2 microglial cell lines using CRISPR-Cas9 gene editing and conducted a series of biological and molecular assays to elu

SUBMITTER: Song XYR 

PROVIDER: S-EPMC12153799 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature