Advanced Paternal Age and Sperm Proteome Dynamics: A Possible Explanation for Age-Associated Male Fertility Decline.

ABSTRACT: Male fertility is strongly influenced by environmental exposures, lifestyle, and advancing age. While advanced paternal age (APA) has been linked with a progressive decline in male fertility, poor reproductive outcomes, and decreased offspring health, the molecular mechanisms underlying these alterations remain unclear. In this work, we investigated the impact of men's age on human sperm protein expression and phosphorylation to identify molecular alterations possibly responsible for the age-associated decline in male fertility. Semen samples from volunteers attending fertility consultations at the Hospital of Aveiro were collected, analyzed according to WHO's guidelines, and processed by the density gradient technique. The proteome and phosphoproteome of 19 normozoospermic human sperm sam

SUBMITTER: Santiago J 

PROVIDER: S-EPMC12153847 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature