Development of Second-Generation Acyl Silane Photoaffinity Probes for Cellular Chemoproteomic Profiling.

ABSTRACT: Deconvolution of the protein targets of hit compounds from phenotypic screens, often conducted in live cells, is critical for understanding mechanism of action and identifying potentially hazardous off-target interactions. While photoaffinity labeling and chemoproteomics are long-established approaches for discovering small-molecule-protein interactions in live cells, there are a relatively small number of photoaffinity labeling strategies that can be applied in intracellular settings. Recently, we reported a novel chemical framework for photoaffinity labeling based on the photo-Brook rearrangement of acyl silanes and demonstrated its ability, when appended to protein-targeting ligands, to label recombinant proteins. Here, we report the application of these probes to live cell photoaffinit

SUBMITTER: Page ACS 

PROVIDER: S-EPMC12154613 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature