A Transcriptomics-Based Computational Drug Repositioning Pipeline Identifies Simvastatin And Primaquine As Novel Therapeutics For Endometriosis Pain.

ABSTRACT:

Introduction and methods

Endometriosis has limited treatment options, prompting the search for novel therapeutics. We previously used a transcriptomics-based computational drug repositioning pipeline to analyze public bulk transcriptomic data and identified several drug candidates. Fenoprofen, our top in silico candidate, was validated in a rat model. Building on this, we now evaluate two additional candidates, simvastatin (a cholesterol-lowering drug) and primaquine (an antimalarial), based on strong gene expression reversal scores and favorable safety profiles. Using a validated rat model of endometriosis and pain, we conducted behavioral testing, bulk RNA sequencing, and differential expression analysis to assess their therapeutic potential.

Results

Of 299 drugs identifie

SUBMITTER: Oskotsky TT 

PROVIDER: S-EPMC12154721 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature