<i>EBSn,</i> a robust synthetic reporter for monitoring ethylene responses in plants.

ABSTRACT: Ethylene is a gaseous plant hormone that controls a wide array of physiologically relevant processes, including plant responses to biotic and abiotic stress, and induces ripening in climacteric fruits. To monitor ethylene in plants, analytical methods, phenotypic assays, gene expression analysis, and transcriptional or translational reporters are typically employed. In the model plant Arabidopsis, two ethylene-sensitive synthetic transcriptional reporters have been described, 5xEBS:GUS and 10×2EBS-S10:GUS. These reporters harbor a different type, arrangement, and number of homotypic cis-elements in their promoters and thus may recruit the ethylene master regulator EIN3 in the context of alternative transcriptional complexes. Accordingly, the patterns of GUS activity in

SUBMITTER: Fernandez-Moreno JP 

PROVIDER: S-EPMC12154813 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature