In-cell Proteomics Enables High-Resolution Spatial and Temporal Mapping of Early <i>Xenopus tropicalis</i> Embryos.

ABSTRACT: Early embryonic development requires tightly regulated molecular programs to coordinate cell division, fate specification, and spatial patterning. While transcriptomic profiling is widely performed, proteomic analyses of early vertebrate embryos remain limited due to technical challenges in embryonic sample preparation. Here, we propose an "in-cell proteomics" approach, which bypasses cell lysis and yolk depletion, processes individual embryos directly in functionalized filter devices, and generates liquid chromatography-mass spectrometry (LC-MS)-friendly samples in an extremely robust and streamlined manner. Combined with a single-shot data-independent acquisition (DIA) MS workflow, this approach enabled us to consistently quantify ~6,200 proteins from a single Xenopus tropicalis e

SUBMITTER: Sun J 

PROVIDER: S-EPMC12154854 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature