TRPV4 modulates substrate stiffness mechanosensing and transcellular pore formation in human Schlemm's canal cells.

ABSTRACT: Pathological changes in the biomechanical environment of Schlemm's canal (SC) inner wall cells, such as substrate stiffening and increased cellular stretch, are associated with ocular hypertension, a key risk factor for the development of glaucoma. Cell membrane stretch can trigger the activation of transient receptor potential vanilloid 4 (TRPV4) mechanosensitive ion channels, allowing calcium influx and initiating downstream signaling. However, the precise role of TRPV4 in SC cell mechanobiology remains unclear. Here, we demonstrate that sustained inhibition of TRPV4 activity modulates substrate stiffness mechanosensing to thereby affect the remodeling of the actin cytoskeleton and extracellular matrix of SC cells. This is accompanied by a reduction in cell stiffness and an increase in t

SUBMITTER: Li H 

PROVIDER: S-EPMC12154856 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature