Multimodal analysis of <i>in vitro</i> hematopoiesis reveals blood cell-specific genetic impacts on complex disease traits.

ABSTRACT: In vitro hematopoiesis systems can be used to define mechanisms for blood cell formation and function, produce cell therapeutics, and model blood cell contributions to systemic disease. Hematopoietic progenitor cell (HPC) production remains inefficient, precluded by knowledge gaps related to specification and morphogenesis of specialized hemogenic endothelial cells, which undergo an endothelial-to-hematopoietic transition (EHT) to form HPCs. We elected to define changes in gene expression and chromatin organization during HPC formation to reveal regulatory mechanisms. Using paired single cell RNA/ATAC sequencing together with Hi-C, we profiled cells before and after EHT. Pathway analysis and pseudotime inferences confirmed a continuum of stromal and endothelial cells undergoing deve

SUBMITTER: Qiu R 

PROVIDER: S-EPMC12154955 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature