Graphical and Interactive Spatial Proteomics Image Analysis Workflow.

ABSTRACT: Spatial proteomics provides a spatially resolved view of protein expression and localization within cells and tissues by mapping the location and abundance of proteins. There is a need for fully-integrated end-to-end imaging workflows for spatial proteomic analysis that are flexible, high-throughput, and support graphical and interactive visualizations. We present a modular and interactive spatial proteomic image analysis workflow with individual steps containerized that empowers biomedical researchers to reproducibly execute and customize complex analyses. Our workflow consists of cell segmentation, unsupervised clustering, validation of clusters on the image, and cell type clustering results visualization. Users can utilize a form-based graphical interface to execute and customize multi-

SUBMITTER: Singh P 

PROVIDER: S-EPMC12154974 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature