Depth-Variant Deconvolution Applied to Widefield Microscopy for Rapid Large-Volume Tissue Imaging.

ABSTRACT: Innovations in 3D tissue imaging have revolutionized research, but limitations stemming from lengthy protocols and equipment accessibility persist. Classical widefield microscopy is fast and accessible but often excluded from 3D imaging workflows due to its lack of optical sectioning. Here we combine tissue clearing with a depth-variant deconvolution approach customized for large-volume widefield imaging to achieve subnuclear axial resolution in tissues to a depth of 500 μm. We illustrate the utility of this method in a mouse model of ileitis and to gain a 3D perspective in thick brain slices from a mouse model of cerebral amyloid angiopathy, where we resolved large and small blood vessels, including those with amyloid deposits, attaining resolution that compared favorably to tile-scanning

SUBMITTER: Lee DD 

PROVIDER: S-EPMC12155202 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature