Global expression profiling of theophylline response genes in macrophages: evidence of airway anti-inflammatory regulation.

ABSTRACT:

Background

Theophylline has been used widely as a bronchodilator for the treatment of bronchial asthma and has been suggested to modulate immune response. While the importance of macrophages in asthma has been reappraised and emphasized, their significance has not been well investigated. We conducted a genome-wide profiling of the gene expressions of macrophages in response to theophylline.

Methods

Microarray technology was used to profile the gene expression patterns of macrophages modulated by theophylline. Northern blot and real-time quantitative RT-PCR were also used to validate the microarray data, while Western blot and ELISA were used to measure the levels of IL-13 and LTC4.

Results

We identified dozens of genes in macrophages that were dose-dependently down- o

SUBMITTER: Yao PL 

PROVIDER: S-EPMC1215521 | biostudies-literature | 2005 Aug

REPOSITORIES: biostudies-literature