Integrated Metabolomics and Network Pharmacology to Reveal the Mechanisms of <i>Forsythia suspensa</i> Extract Against Respiratory Syncytial Virus.

ABSTRACT: To investigate the therapeutic impact of Forsythia suspensa extract (FS) on RSV-infected mice and explore its antiviral pharmacodynamic foundations. Methods: An integrated analytical approach, combining UPLC-Q-TOF/MS with network pharmacology, was employed to analyze and identify the chemical constituents in FS, particularly those exhibiting antiviral properties against RSV. The study integrated network pharmacology and metabolomics for further analysis, and molecular docking and interaction experiments were conducted to validate the pharmacodynamic mechanisms. Finally, an RSV pneumonia mouse model was employed to evaluate the therapeutic influence of FS, including pathological and immunohistochemistry assessments. Twenty-five components in FS were identified through UPLC-Q-TOF/MS a

SUBMITTER: Du H 

PROVIDER: S-EPMC12155444 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature