The staphylococcal collagen adhesin CNA35 effectively detects collagen and its fragments in blots after SDS-PAGE.

ABSTRACT: Collagens are a diverse family of proteins present in the extracellular matrix (ECM) of all animals. They play crucial roles in providing structural support to tissues, forming scaffolds for ECM suprastructures, and signaling cells. Certain collagen-binding proteins from pathogenic bacteria, such as CollageN Adhesin (CNA) from Staphylococcus aureus, interact with the collagen triple helix to promote host invasion. The extracellular portion of CNA, known as CNA35, which has a molecular weight of 35 kDa, has been used for in vitro, ex vivo, and in vivo staining of various collagen types in tissues. Detecting various types of collagens necessitates the use of type- and specie-specific antibodies, which typically exhibit weak affinities for the triple helical region

SUBMITTER: Pokidysheva EN 

PROVIDER: S-EPMC12156248 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature