Phytochemical Characterization, Antioxidant Activity, and Anti-Melanoma Mechanism of Flower Buds of <i>Magnolia biondii</i> Pamp.

ABSTRACT: The flower buds of Magnolia biondii Pamp. (MBP), one of the botanical sources of Xinyi (Flos Magnoliae), are widely used in traditional medicine; however, their potential role in melanoma treatment remains unexplored. In this study, the phytochemical composition, antioxidant activity, and anti-melanoma mechanisms of MBP extracts were systematically investigated. Phytochemical profiling using UHPLC-Q-Exactive Orbitrap MS identified 26 bioactive compounds. The ethanol extract exhibited high total flavonoid and polyphenol contents, correlating with enhanced antioxidant capacity as demonstrated by DPPH and ABTS assays. Network pharmacology analysis highlighted the JAK/STAT signaling pathway, identifying STAT3 and STAT1 as core targets. Western blot analysis confirmed MBP s

SUBMITTER: Li S 

PROVIDER: S-EPMC12157075 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature