Overexpressing <i>BrWRKY22</i> Delays Flowering and Leaf Senescence via Inhibition of GA Biosynthesis in <i>Brassica rapa</i>.

ABSTRACT: WRKY transcription factors play a predominant role in plant stress responses, as well as growth and development. Although WRKY genes have been extensively studied in model plants, little is known about them in Brassica rapa. In this study, the BrWRKY22 gene was isolated and characterized. BrWRKY22 is nuclear localized and has self-activation and dimerization activity. BrWRKY22 was highly expressed in young leaves, roots, and stems. The overexpressed BrWRKY22 Arabidopsis and Brassica rapa lines exhibited a dwarfish, delayed flowering and leaf senescence phenotype compared to the wild-type (WT). Molecular evidence showed that the transcript levels of BrCHLP are increased, whereas those of BrLFY, BrSOC1, BrGA20OX2, BrGA3

SUBMITTER: Uddin JBG 

PROVIDER: S-EPMC12157233 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature