VesicleVoyager: In vivo selection of surface displayed proteins that direct extracellular vesicles to tissue-specific targets.

ABSTRACT: The development of technologies for screening proteins that bind to specific tissues in vivo and facilitate delivery of large cargos remains challenging, with most approaches limited to cell culture systems that often yield clinically irrelevant hits. To overcome this limitation, we developed a novel molecular screening platform using an extracellular vesicle (EV) display library. EVs are natural molecular carriers capable of delivering diverse cargos, which can be engineered to enhance specificity and targeting through surface modifications. We constructed an EV-display library presenting monobody repertoires on EV surfaces, with genetic cargo inside the EVs corresponding to the displayed proteins. These libraries were screened for tissue specific delivery through serial passage in mice v

SUBMITTER: Kawai-Harada Y 

PROVIDER: S-EPMC12157350 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature