A spatial transcriptomic atlas of acute neonatal lung injury across development and disease severity.

ABSTRACT: A molecular understanding of lung organogenesis requires delineation of the timing and regulation of the cellular transitions that ultimately form and support a surface capable of gas exchange. While the advent of single-cell transcriptomics has allowed for the discovery and identification of transcriptionally distinct cell populations present during lung development, the spatiotemporal dynamics of these transcriptional shifts remain undefined. With imaging-based spatial transcriptomics, we analyzed the gene expression patterns in 17 human infant lungs at varying stages of development and injury, creating a spatial transcriptomic atlas of ~1.2 million cells. We applied computational clustering approaches to identify shared molecular patterns among this cohort, informing how tissue architec

SUBMITTER: Mallapragada S 

PROVIDER: S-EPMC12157373 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature