Precise MRI-Histology Coregistration of Paraffin-Embedded Tissue with Blockface Imaging.

ABSTRACT: Magnetic resonance imaging (MRI) provides 3D spatial information on tissue, yet it lacks at the molecular level. In contrast, histology provides cellular and molecular information, but it lacks the 3D spatial context and direct in vivo translation. Coregistering the two is key for the 3D-embedding of histological details, validating pathological MRI findings, and finding quantitative imaging biomarkers of neurodegenerative diseases. However, coregistration is challenging due to non-linear distortions of the tissue from histological processing and sectioning leading to microscopic and macroscopic nonlinear 3D deformations between specimen MRI and stained histology sections. To address this, we developed a novel pipeline, named Brewster's Blockface Quantification (BBQ), integrating ro

SUBMITTER: Wang Y 

PROVIDER: S-EPMC12157374 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature