An updated and spatially validated somatic single-cell atlas of <i>Hydractinia symbiolongicarpus</i>.

ABSTRACT: Single-cell RNA sequencing (scRNA-seq) has revolutionized transcriptomic research, enabling the creation of detailed tissue, organ, and species-level atlases for model organisms. In Hydractinia, a cnidarian model for stem cell and regeneration studies, recent atlases have revealed key insights into cell types and developmental processes. However, these atlases remain limited in cell numbers and transcriptomic depth and cell type assignments were largely made in silico. Here, we present an updated Hydractinia single-cell atlas by integrating new datasets from fixed cells with previously published live-cell data. This expanded atlas captures over 47,000 cells from feeding polyps and stolon tissue, recovering and refining major somatic cell lineages including cnidocytes,

SUBMITTER: Song J 

PROVIDER: S-EPMC12157527 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature