TITAN-RNA: A hybrid-capture sequencing panel detects known and unknown <i>Flaviviridae</i> for diagnostics and vector surveillance.

ABSTRACT: Clinical testing and public health surveillance can be significantly improved by incorporating sequencing-based molecular detection and subtyping for real-time monitoring of virus evolution. With phylogenetic analysis used for speciation and variant subtyping, target analyte specificity can be relaxed well beyond typical parameters acceptable in PCR-based diagnostics. Hybrid capture is a promising way to enrich large numbers of sequences with maximal flexibility, using standard molecular biology laboratory equipment and small benchtop sequencers. Here, we report the development and bench validation of a hybrid capture based next-generation sequencing diagnostic panel for all known viral tick-borne pathogens, TITAN-RNA. Based on systematic testing with simulated novel viruses and field samp

SUBMITTER: Murrell CM 

PROVIDER: S-EPMC12157608 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature