Cell-surface proteomic profiling identifies CD72 as a regulator of microglial tiling.

ABSTRACT: Microglial tiling-the phenomenon of consistent cell-to-cell distances and non-overlapping processes-is regarded as a qualitative indicator of homeostasis, but mechanisms of microglial tiling are unknown. We used cell-surface proximity labeling and mass spectrometry to profile the microglial cell-surface proteome in an in vitro model of homeostatic glial physiology and used single-cell RNA sequencing and public databases to identify candidate cell-surface proteins that might modulate tiling. We designed an image-based functional assay which measures six morphological/spatial readouts to screen these proteins for modulation of tiling. CD72, a coreceptor to the B cell receptor that is expressed by microglia, disrupted tiling; we validated its effects in vitro and in situ

SUBMITTER: Chan TC 

PROVIDER: S-EPMC12157663 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature