Kinesin-1 Autoinhibition Tunes Cargo Transport by Motor Ensembles.

ABSTRACT: Intracellular vesicular transport by kinesin-1 motors through numerous 3-dimensional (3D) microtubule (MT) intersections must be regulated to support proper vesicle delivery. Knowing kinesin-1 can be regulated via autoinhibition, does kinesin-1 exhibit autoinhibition on cargo, and could this regulate vesicular transport through 3D MT intersections in vitro? To answer this question, we compared liposome transport by ~10 nearly full-length kinesin-1 motors with KLC bound (KinΔC) versus constitutively active control (K543). In 3D MT intersections, KinΔC-liposomes terminate (48%), go straight (43%), but rarely turn (9%), starkly contrasting K543-liposomes which go straight (57%), turn (31%), but rarely terminate (12%). On single MTs, KinΔC-liposomes have reduced run lengths and detachme

SUBMITTER: Bensel BM 

PROVIDER: S-EPMC12157665 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature