A haplotype-resolved view of human gene regulation.

ABSTRACT: Diploid human cells contain two non-identical genomes, and differences in their regulation underlie human development and disease. We present Fiber-seq Inferred Regulatory Elements (FIRE) and show that FIRE provides a more comprehensive and quantitative snapshot of the accessible chromatin landscape across the 6 Gbp diploid human genome, overcoming previously known and unknown biases afflicting our existing regulatory element catalog. FIRE provides a comprehensive genome-wide map of haplotype-selective chromatin accessibility (HSCA), exposing novel imprinted elements that lack underlying parent-of-origin CpG methylation differences, common and rare genetic variants that disrupt gene regulatory patterns, gene regulatory modules that enable genes to escape X chromosome inactivation, and auto

SUBMITTER: Vollger MR 

PROVIDER: S-EPMC12157683 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature