Identification of three novel mite allergens, Der f 42, Der f 43, and Der f 44, from <i>Dermatophagoides farinae</i> by gelsolin interactome analysis.

ABSTRACT:

Background

House dust mites (HDMs) produce major inhaled allergens that trigger allergic diseases worldwide. The identities of the full spectrum of HDM allergenic components are not yet known. We aimed to develop a new gelsolin interactome-analysis (GIA) method for discovering and identifying novel allergens.

Methods

Gelsolin-binding proteins (GBPs) in Dermatophagoides farinae extracts were analyzed with gelsolin-affinity resin and LC-MS/MS (liquid chromatography coupled to tandem mass spectrometry) analyses. Recombinant proteins generated from cDNAs encoding candidate allergens were expressed in a prokaryotic system. IgE binding was evaluated by ELISA (enzyme-linked immunosorbent assay), western blotting, and dot-blotting.

Results

A total of 14 GBPs were assay

SUBMITTER: Cai ZL 

PROVIDER: S-EPMC12158493 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature