IDO1 improves postischemic neovascularization in aged mice by boosting endothelial NAD⁺ de novo synthesis and curbing endothelial senescence.

ABSTRACT:

Introduction

Peripheral arterial disease (PAD) is prevalent among the elderly, and therapeutic neovascularization is a research hotspot in PAD treatment. Supplementing nicotinamide adenine dinucleotide (NAD⁺) precursors is an important approach for promoting neovascularization, but clinical trials in the elderly PAD patients have shown limited success. This study aims to find effective ways to boost NAD⁺ levels in elderly PAD patients to enhance neovascularization.

Methods

Transcriptome and NAD⁺-targeted metabolomics analyses were conducted on ischemic hindlimb muscle endothelial cells (ECs). The role of indoleamine 2,3-dioxygenase 1 (IDO1) in postischemic neovascularization was studied using global knockout mice. Mechanisms regulating IDO1 e

SUBMITTER: Chen M 

PROVIDER: S-EPMC12158617 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature