RDT performance through high-throughput bead-based antigen detection during malaria school survey in Senegal.

ABSTRACT:

Background

Rapid Diagnostic Tests (RDTs) remain the frontline tool for malaria diagnosis, but their performance in detecting low-density infections is variable and poorly characterized at the population level.

Objective

This study aimed to evaluate the diagnostic performance of HRP2-based RDTs by integrating high-throughput bead-based HRP2 quantification into school-based malaria surveys.

Methods

A cross-sectional study was conducted in three Senegalese districts (Diourbel, Tambacounda, and Kédougou), enrolling 3,748 school-aged children. All participants were tested using RDTs, and dried blood spots were analyzed with a multiplex bead-based HRP2 assay. A Gaussian mixture model was used to classify HRP2 positivity, and logistic regression assessed the relationship bet

SUBMITTER: Diallo MA 

PROVIDER: S-EPMC12159022 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature