METTL3-mediated SNHG1 m⁶A modification promotes proliferation and migration through transcriptional regulation of WDR74 in osteosarcoma.

ABSTRACT:

Introduction

As the most prevalent internal RNA modification in eukaryotic transcripts, N6-methyladenosine (m⁶A) which is catalyzed by methyltransferase-like 3 (METTL3), is widely involved in cancerous diseases. However, the role of METTL3 and small nucleolar RNA host gene 1 (SNHG1) playing in osteosarcoma (OS) remains largely unknown.

Methods

Bioinformatics analysis, RT-qPCR, western blotting assays were used to detect the expression of METTL3, SNHG1, RNA binding motif protein 15 (RBM15), WD repeat domain 74 (WDR74) and EWS RNA binding protein 1 (EWSR1) accordingly. Cell proliferation and motility ability changes were assessed by colony formation and transwell migration assays. RNA stability changes were evaluated by an actinomycin D assay. The level of SN

SUBMITTER: Qiu G 

PROVIDER: S-EPMC12159053 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature