Isolation and characterization of a novel coltivirus from <i>Haemaphysalis concinna</i> ticks in Northeastern China.

ABSTRACT:

Backgroud

Coltiviruses are spherical, non-enveloped viruses with 12 double-stranded RNA segments, belonging to the family Spinareoviridae, and predominantly transmitted by ticks. This study isolated and characterized a novel coltivirus, designated Woodland tick reovirus (WLTRV), from Haemaphysalis concinna ticks collected in Helong City, Jilin Province, in Northeastern China.

Methods

SW-13 and Vero cells were used to isolate WLTRV through three blind passages, while seven mammalian cell lines assessed viral growth. Viral morphology was observed by electron microscopy. Next-generation sequencing, 5' and 3' rapid amplification of cDNA ends were used to determine WLTRV whole genome sequences, and phylogenetic methods were used to characterize WLTRV. A real-time qu

SUBMITTER: Yang YH 

PROVIDER: S-EPMC12159217 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature