Platelet-rich fibrin-conditioned medium promotes osteogenesis of dental pulp stem cells through TGF-β and PDGF signaling.

ABSTRACT:

Introduction

Mesenchymal stem cells (MSCs), a key cell source for regenerative medicine, are conventionally cultured in human autologous serum (HAS) or fetal bovine serum (FBS). Platelet-rich fibrin-conditioned medium (PRF-CM) has emerged as a potential alternative to FBS, promoting the osteogenic differentiation of dental pulp stem cells (DPSCs) more efficiently than FBS. However, the molecular mechanisms underlying PRF-CM-induced osteogenesis in DPSCs remain unclear. Therefore, the aim of this study was to elucidate these mechanisms.

Methods

PRF-CM was prepared from peripheral blood samples collected from three healthy donors. DPSCs were derived from the dental pulp extracted from the third molars of three healthy donors. The mRNA expression patterns of DPSCs cultured in F

SUBMITTER: Fujii Y 

PROVIDER: S-EPMC12159295 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature