Spermidine suppresses DC activation via eIF5A hypusination and metabolic adaptation.

ABSTRACT:

Introduction

Cell metabolism plays an important role in immune effector responses and through responding to metabolic signals, immune cells can adapt and regulate their function. Arginine metabolism in dendritic cells (DC) has been shown to reduce T cell activation; however, it is unclear how this immunosuppressive state is induced.

Method

To address this issue, we examined the immunomodulatory capacity of various metabolites from arginine metabolism.

Results

Through the use of a recently described DC:T cell interaction assay and flow cytometry we demonstrated that spermidine most significantly inhibited DC activation, preventing subsequent interactions with CD4 T cells. DC function could be restored by addition of inhibitors of spermidine metabolism via the eIF5A-hyp

SUBMITTER: Meehan GR 

PROVIDER: S-EPMC12159527 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature