Proteomics Reveals Increased Periostin in Synovial Fluid From Canine and Human Anterior Cruciate Ligament Injury.

ABSTRACT: Posttraumatic osteoarthritis (PTOA) is a common sequela to anterior cruciate ligament (ACL) injury in humans and dogs. Identification of conserved targets between species may hasten biomarker identification and therapeutic development. To identify differentially regulated synovial fluid (SF) proteins in ACL injury, SF samples were collected from knees with ACL injury or control knees (n = 8 per species) in human and dog patients. The SF proteome was evaluated using nano-scale reverse-phase chromatography and tandem mass spectrometry. Associations between proteins and ACL injury were analyzed in both species. Immunoassays were used to validate key proteins identified via mass spectrometry, including periostin, α-2-macroglublin, and lubricin, in additional SF samples (dog: n = 57 ACL, n = 31

SUBMITTER: Womack SJ 

PROVIDER: S-EPMC12159583 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature